Further evidence of a cybridization requirement for plant regeneration from citrus leaf protoplasts following somatic fusion
Identifieur interne : 003498 ( Main/Exploration ); précédent : 003497; suivant : 003499Further evidence of a cybridization requirement for plant regeneration from citrus leaf protoplasts following somatic fusion
Auteurs : J. W. Grosser [États-Unis] ; F. G. Jr Gmitter [États-Unis] ; N. Tusa ; G. Reforgiato Recupero ; P. CucinottaSource :
- Plant cell reports [ 0721-7714 ] ; 1996.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Agrume.
English descriptors
- KwdEn :
Abstract
Somatic hybridization experiments in Citrus that involve the fusion of protoplasts of one parent isolated from either nucellus-derived embryogenic callus or suspension cultures with leaf-derived protoplasts of a second parent, often result in the regeneration of diploid plants that phenotypically resemble the leaf parent. In this study, plants of this type regenerated following somatic fusions of the following three parental combinations were analyzed to determine their genetic origin (nuclear and organelle): (embryogenic parent listed first, leaf parent second) (1) calamondin (C. microcarpa Bunge) + 'Keen' sour orange (C. aurantium L.), (2) Cleopatra mandarin (C. reticulata Blanco) + sour orange, and (3) 'Valencia' sweet orange (C. sinensis (L.) Osbeck) + 'Femminello' lemon (C. limon (L.) Burm. f.). Isozyme analyses of PGI, PGM, GOT, and IDH zymograms of putative cybrid plants, along with RFLP analyses using a nuclear genome-specific probe showed that these plants contained the nucleus of the leaf parent. RFLP analyses using mtDNA-specific probes showed that these plants contained the mitochondrial genome of the embryogenic callus donor, thereby confirming cybridization. RFLP analyses using cpDNA-specific probes revealed that the cybrid plants contained the chloroplast genome of either one or the other parent. These results support previous reports indicating that acquisition of the mitochondria of embryogenic protoplasts by leaf protoplasts is a prerequisite for recovering plants with the leaf parent phenotype via somatic embryogenesis following somatic fusion.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Further evidence of a cybridization requirement for plant regeneration from citrus leaf protoplasts following somatic fusion</title><author><name sortKey="Grosser, J W" sort="Grosser, J W" uniqKey="Grosser J" first="J. W." last="Grosser">J. W. Grosser</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>University of Florida, IFAS, Citrus Research and Education Center, 700 Experiment Station Road</s1><s2>Lake Alfred, FL 33850</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Gmitter, F G Jr" sort="Gmitter, F G Jr" uniqKey="Gmitter F" first="F. G. Jr" last="Gmitter">F. G. Jr Gmitter</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>University of Florida, IFAS, Citrus Research and Education Center, 700 Experiment Station Road</s1><s2>Lake Alfred, FL 33850</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Tusa, N" sort="Tusa, N" uniqKey="Tusa N" first="N." last="Tusa">N. Tusa</name></author><author><name sortKey="Reforgiato Recupero, G" sort="Reforgiato Recupero, G" uniqKey="Reforgiato Recupero G" first="G." last="Reforgiato Recupero">G. Reforgiato Recupero</name></author><author><name sortKey="Cucinotta, P" sort="Cucinotta, P" uniqKey="Cucinotta P" first="P." last="Cucinotta">P. Cucinotta</name></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">96-0262246</idno><date when="1996">1996</date><idno type="stanalyst">PASCAL 96-0262246 INIST</idno><idno type="RBID">Pascal:96-0262246</idno><idno type="wicri:Area/PascalFrancis/Corpus">000B68</idno><idno type="wicri:Area/PascalFrancis/Curation">001341</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000B54</idno><idno type="wicri:doubleKey">0721-7714:1996:Grosser J:further:evidence:of</idno><idno type="wicri:Area/Main/Merge">003756</idno><idno type="wicri:Area/Main/Curation">003498</idno><idno type="wicri:Area/Main/Exploration">003498</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Further evidence of a cybridization requirement for plant regeneration from citrus leaf protoplasts following somatic fusion</title><author><name sortKey="Grosser, J W" sort="Grosser, J W" uniqKey="Grosser J" first="J. W." last="Grosser">J. W. Grosser</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>University of Florida, IFAS, Citrus Research and Education Center, 700 Experiment Station Road</s1><s2>Lake Alfred, FL 33850</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Gmitter, F G Jr" sort="Gmitter, F G Jr" uniqKey="Gmitter F" first="F. G. Jr" last="Gmitter">F. G. Jr Gmitter</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>University of Florida, IFAS, Citrus Research and Education Center, 700 Experiment Station Road</s1><s2>Lake Alfred, FL 33850</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Tusa, N" sort="Tusa, N" uniqKey="Tusa N" first="N." last="Tusa">N. Tusa</name></author><author><name sortKey="Reforgiato Recupero, G" sort="Reforgiato Recupero, G" uniqKey="Reforgiato Recupero G" first="G." last="Reforgiato Recupero">G. Reforgiato Recupero</name></author><author><name sortKey="Cucinotta, P" sort="Cucinotta, P" uniqKey="Cucinotta P" first="P." last="Cucinotta">P. Cucinotta</name></author></analytic><series><title level="j" type="main">Plant cell reports</title><title level="j" type="abbreviated">Plant cell rep.</title><idno type="ISSN">0721-7714</idno><imprint><date when="1996">1996</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Plant cell reports</title><title level="j" type="abbreviated">Plant cell rep.</title><idno type="ISSN">0721-7714</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell culture</term><term>Cell fusion</term><term>Citrus aurantium</term><term>Citrus fruit</term><term>Citrus limonum</term><term>Citrus reticulata</term><term>Citrus sinensis</term><term>Nucleocytoplasmic hybrid</term><term>Plant leaf</term><term>Protoplast</term><term>Regeneration</term><term>Somatic hybrid</term><term>Suspension culture</term><term>Vegetals</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Hybride somatique</term><term>Culture en suspension</term><term>Protoplaste</term><term>Régénération</term><term>Culture cellulaire</term><term>Citrus aurantium</term><term>Citrus limon</term><term>Citrus sinensis</term><term>Citrus reticulata</term><term>Fusion cellulaire</term><term>Feuille végétal</term><term>Hybride nucléocytoplasmique</term><term>Végétal</term><term>Agrume</term><term>Citrus microcarpa</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Agrume</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Somatic hybridization experiments in Citrus that involve the fusion of protoplasts of one parent isolated from either nucellus-derived embryogenic callus or suspension cultures with leaf-derived protoplasts of a second parent, often result in the regeneration of diploid plants that phenotypically resemble the leaf parent. In this study, plants of this type regenerated following somatic fusions of the following three parental combinations were analyzed to determine their genetic origin (nuclear and organelle): (embryogenic parent listed first, leaf parent second) (1) calamondin (C. microcarpa Bunge) + 'Keen' sour orange (C. aurantium L.), (2) Cleopatra mandarin (C. reticulata Blanco) + sour orange, and (3) 'Valencia' sweet orange (C. sinensis (L.) Osbeck) + 'Femminello' lemon (C. limon (L.) Burm. f.). Isozyme analyses of PGI, PGM, GOT, and IDH zymograms of putative cybrid plants, along with RFLP analyses using a nuclear genome-specific probe showed that these plants contained the nucleus of the leaf parent. RFLP analyses using mtDNA-specific probes showed that these plants contained the mitochondrial genome of the embryogenic callus donor, thereby confirming cybridization. RFLP analyses using cpDNA-specific probes revealed that the cybrid plants contained the chloroplast genome of either one or the other parent. These results support previous reports indicating that acquisition of the mitochondria of embryogenic protoplasts by leaf protoplasts is a prerequisite for recovering plants with the leaf parent phenotype via somatic embryogenesis following somatic fusion.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Floride</li></region></list><tree><noCountry><name sortKey="Cucinotta, P" sort="Cucinotta, P" uniqKey="Cucinotta P" first="P." last="Cucinotta">P. Cucinotta</name><name sortKey="Reforgiato Recupero, G" sort="Reforgiato Recupero, G" uniqKey="Reforgiato Recupero G" first="G." last="Reforgiato Recupero">G. Reforgiato Recupero</name><name sortKey="Tusa, N" sort="Tusa, N" uniqKey="Tusa N" first="N." last="Tusa">N. Tusa</name></noCountry><country name="États-Unis"><region name="Floride"><name sortKey="Grosser, J W" sort="Grosser, J W" uniqKey="Grosser J" first="J. W." last="Grosser">J. W. Grosser</name></region><name sortKey="Gmitter, F G Jr" sort="Gmitter, F G Jr" uniqKey="Gmitter F" first="F. G. Jr" last="Gmitter">F. G. Jr Gmitter</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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